منابع مشابه
DNA Fragmentation in Cultured Cells Exposed to High Linear Energy Transfer Radiation
Höglund, E. 2000. DNA fragmentation in cultured cells exposed to high linear energy transfer radiation. Acta Universitatis Upsaliensis. Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 960. 58 pp. Uppsala. ISBN 91–554–4822–4. The DNA double-strand break (DSB) is a critical lesion which, if not completely restored, can have serious biological consequences. The relati...
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To determine the osteoblastic dysfunction that may be involved in the pathophysiology of osteoporosis in men we have compared histomorphometric indices of bone formation with in vitro characteristics of osteoblastic cells isolated from the trabecular bone surface in 23 untreated men with eugonadal osteoporosis. In most patients (n = 14), trabecular bone loss resulted from decreased bone formati...
متن کاملNew perspectives into bacterial DNA transfer to human cells.
The type IV secretion system (T4SS) VirB/D4 of the facultative intracellular pathogen Bartonella henselae is known to translocate bacterial effector proteins into human cells. Two recent reports on DNA transfer into human cells have demonstrated the versatility of this bacterial secretion system for macromolecular substrate transfer. Moreover, these findings have opened the possibility for deve...
متن کاملGene Transfer into Sub- cultured Endometrial Cells Using Lipofection
Lipofection using the Lipofectin® reagent was optimized to transiently transfect subcultured guinea pig endometrial stromal cells with a β-galactosidase gene driven by a simian virus 40 promoter. Efficient transfection was obtained in the following conditions: a value of six for the ratio of lipofectin to DNA, a low cellular density (105 cells per 35-mm well) at the time of subculture (48 h bef...
متن کاملdna methylation and histone acetylation patterns in cultured bovine adipose tissue-derived stem cells (badscs)
objective: many studies have focused on the epigenetic characteristics of donor cells to improve somatic cell nuclear transfer (scnt). we hypothesized that the epigenetic status and chromatin structure of undifferentiated bovine adipose tissue-derived stem cells (badscs) would not remain constant during different passages. the objective of this study was to determine the mrna expression pattern...
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ژورنال
عنوان ژورنال: The American Journal of Human Genetics
سال: 1999
ISSN: 0002-9297
DOI: 10.1086/302359